Large plasmids associated with virulence in Shigella species have a common function necessary for epithelial cell penetration.
نویسندگان
چکیده
Large plasmids (120 to 140 megadaltons) associated with virulence of Shigella sonnei, S. flexneri 2a and S. dysenteriae 1 were transferred from each strain into Escherichia coli K-12 and avirulent S. flexneri 1b strains by ampicillin transposon (Tn1)-mediated conduction. Strains with the virulence plasmid could penetrate tissue culture cells irrespective of the original host of the plasmid.
منابع مشابه
Virulence-associated genetic regions comprising 31 kilobases of the 230-kilobase plasmid in Shigella flexneri 2a.
By random transposon Tn5 insertions, we previously identified six virulence-associated SalI fragments, B, D, F, G, H, and P, in the 230-kilobase plasmid pMYSH6000 of Shigella flexneri 2a. In this study, we analyzed the sites of 134 independent Tn5 insertions on four contiguous SalI fragments, B, P, H, and D, of pMYSH6000 and identified five virulence-associated regions; four were associated wit...
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عنوان ژورنال:
- Infection and immunity
دوره 48 1 شماره
صفحات -
تاریخ انتشار 1985